Evaluation of DNA expression of nuclear factor kB (NF-kB) in oral squamous cell carcinoma patients using RT-PCR

Backgrounds: Cancer is a fatal disease caused mainly by environmental factors that mutategenes encoding critical cell-regulatory proteins. Oral cavity squamous cell carcinoma (OSCC) isthe sixth most common cancer in the world. Degradation of the cell cycle and the proliferation ofmalignant cells results in the loss of control mechanisms that ensure the normal function oftissues. Traditional risk factors of oral cancer include tobacco and alcohol abuse. The nuclearfactor kB (NF-kB) comprises a family of transcription factors regulates the expression of genesinvolved in many processes that play a key role in the development and progression of cancersuch as proliferation, migration and apoptosis and growing evidences support a major role inoncogenesis. Aberrant or constitutive NF-kB activation has been detected in many humanmalignancies. In the present study, we evaluated the expression of the NF-kB genes in OSCCpatients and normal controlsMaterials and Methods: Samples included ۱۹ paraffin embedded case and ۲۰ biopsy control.RNA from case samples was extracted using high pure FFPE RNA micro kit (ROCHE) andRNA from control sample was extracted using Cinnapure RNA tissue kit. RNAs exchange tocDNA by RevertAid First Strand cDNA Synthesis Kit (fermentase). To evaluate the expressionof NF-kB real-time PCR was performed.Results: Results showed significant differences between OSCC patient and healthy groups forexpression of NF-kB gene (p <۰.۰۳۶) and we saw up regulation of this gene.Conclusion: Our results, for the first time, provide methylation profiles of NF-kB gene in asample of patients with OSCC in a Southeast Iranian population. Up-regulation of NF-kB genehas significant function in oral squamous cell carcinoma. This may provide another promisingtarget for drug development.

کلیدواژه ها:

OSCC ، NF-kB ، Oncogenesis