In-silico analysis of Rac۱ gene and hsa-mir-۱۸۲-۵p and its related target gene of Liver Cancer

Background: Liver cancer (LC) is one of the most invasive malignancies and may derive fromdifferent types of liver cells with an estimated more than ۷۸۱۰۰۰ deaths in ۲۰۱۸. The Rho-familyGTPase Rac۱ plays a key role in carcinogenesis and inflammatory responses. Epidemiologicalstudies demonstrated a relation between chronic inflammation and cancer. Most cases ofhepatocellular carcinoma (approximately ۸۰ %) are associated with cirrhosis related to chronichepatitis following viral infections.Materials and Methods: Based on bioinformatics analysis, hsa-mir-۱۸۲-۵p was selected. Usingby Mirwalk database, Prediction data of genes was collected. Then in the NCBI database, atUniGene, we have checked genes expression. Functional annotation analysis has been parsedwith David database. Pathways relations were collected at KEGG in David database.Results: ۴۱۵ genes were identified for LC. Information of gene expression shows that Rac۱genes were down-expression in normal livers. In the other hand, hsa-miR-۱۸۲-۵p had upexpression.Conclusion: These results suggest that Rac۱ may be a new gene therapy target for LC. BlockingRac۱ expression in LC cells induces apoptosis of these tumor cells and may thus represent a newtherapeutic approach.

کلیدواژه ها:

Liver Cancer ، microRNA ، RAC۱ ، has-miR-۱۸۲