Arash Moradi - Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Shahla Mohammad Ganji - Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Kamal Mohammadian - Department of Radiation Oncology, Hamedan University of Medical Sciences, Mahdieh center, Hamadan, Iran
Aida Alizamir - Department of Pathology, Hamadan University of Medical Sciences, Hamadan, Iran

Background: DNA methylation and long non-coding RNAs (lncRNAs) are the epigeneticmechanisms that numerous investigators studied. Genetic and epigenetic alterations, lead todisruptions in the cellular hemostasis that lead to carcinogenesis. The changes in DNAmethylation, rationally, are not a random process and can accurately characterize the cancerstatus. Recently, the function of lncRNAs has been focused on, and numerous studies try todecipher the potential roles of lncRNAs in cancer biology. This study investigated the correlationbetween DNA methylation and RNA expression of colorectal cancer (CRC).Materials and Methods: The data were investigated at the GEO database, including themethylation array and the expression array of the CRC. Afterward, via the GEO۲R, data wasanalyzed. Consequently, data were evaluated by statistics tests, including Spearman, Pearson,one -way Anova.Results: This bioinformatic-based study found that HAND۲-AS۱ was markedly downregulatedin CRC tissues (p= ۸.۵۲E-۰۵). This downregulation is correlated with the hypermethylation ofthe HAND۲-AS۱ promoter (Spearman: -۰.۴۲ (p = ۵.۸۵E-۱۷), Pearson: -۰.۴۰ (p = ۱.۳۶E-۱۵)).Noteworthy, HAND۲-AS۱could sponges numerous miRNAs, which potentially promote cancerprogression.Conclusion: In this study, the importance of DNA methylation was emphasized. Also, these datashowed that the expression of HAND۲-AS۱ is controlled by DNA methylation, which is alteredin cancer creation.

lncRNA, bioinformatics, HAND۲-AS۱, DNA methylation