Aberrant expression of circ-۰۰۷۲۳۰۹ in breast carcinoma as a promising biomarker for diagnosis
- سال انتشار: 1400
- محل انتشار: کنفرانس بین المللی ژنتیک و ژنومیکس انسانی
- کد COI اختصاصی: CHGGE01_284
- زبان مقاله: انگلیسی
- تعداد مشاهده: 290
نویسندگان
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Genetics Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
چکیده
Backgrounds: Breast cancer (BC) is one of the most common cancers and the second highestmortality in women worldwide. Different studies have shown that there is a close correlationbetween circRNAs and initiation and progression of different cancers and diseases; however,knowledge about the correlation between non-coding RNAs, especially the circRNAs and BC, isnot fully explored. In this study, the expression level of circ-۰۰۷۲۳۰۹ (LIFR) in BC wasinvestigated experimentally and miRNA target genes for it were proposed.Materials and Methods: BC tissue specimens (twenty-nine causes) and their correspondingadjacent normal tissue were collected during the surgery and has-circ-۰۰۷۲۳۰۹ expression inbreast cancer tissues was analyzed using qRT-PCR. A series of functional experiments werecarried out to investigate has-circ-۰۰۷۲۳۰۹ function in breast cancer development. Additionally,miRNAs that potentially target has-circ-۰۰۷۲۳۰۹ were predicted by bioinformatics method.miRTargetlink database (https://ccb-web.cs.uni-saarland.de/mirtargetlink) and DIANA database(http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=mirpath/index/) were used topredict interactions of miRNAs with LIFR.Results: The lower expression levels of circ-۰۰۷۲۳۰۹ in BC tissues was showed compared topaired adjacent normal tissues with P value (P < ۰.۰۰۲). The area under the receiver operatingcharacteristic (ROC) curve was ۰.۷۶۸. In addition, a total ۱۰ miRNAs that can be targeted bycandidate circRNA was predicted base on bioinformatics databases. Moreover, miRNAsselection tools predicted that miR-۱۲۵a-۵p and miR-۲۰۳a-۳p can be targeted by circ-۰۰۷۲۳۰۹.Conclusion: Our findings revealed that the has-circ-۰۰۷۲۳۰۹ plays an essential role in BCprogression and circ-۰۰۷۲۳۰۹ could serve as a prognostic biomarker of BC.کلیدواژه ها
Circular RNA, microRNA, Breast cancer, DIANA database, miRTargetlink databaseاطلاعات بیشتر در مورد COI
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