Over expression of LncRNA DUXAP۸ in gastric cancer tumoral tissues

Backgrounds: Gastric cancer remains fifth most common cancer and often diagnosed at anadvanced stage and is the second leading cause of cancer-related death worldwide. An increasingnumber of studies have found that non-coding RNAs including miRNA and LncRNA playimportant roles in gastric cancer progression. Recent studies highlight pseudogene derived longnon-coding RNAs (LncRNAs) as key regulators of cancer process. However, few of them havebeen well characterized in gastric cancer. Here, we aimed to identify the association betweenpseudogene derived LncRNA DUXAP۸ and growth of gastric cancer cells and also examined theexpression level of LncRNA DUXAP۸ and its association with clinico pathologicalcharacteristic in GC.Materials and Methods: Total RNA of ۱۰۰ specimen’s tumor tissues and ۱۰۰ specimens ofmarginal tissues were extracted by TRIzol reagent. After cDNA synthesis, qRT-PCR wasperformed to evaluate the expression level of DUXAP۸ and the obtained data were analyzed bySPSS ۱۶.۰ and Graph Pad Prism.Results: The relative expression level of DUXAP۸ was significantly increased in tumoral tissuesas compared with the marginal tissues. The AUC index of LncRNA DUXAP۸ was ۰.۶۷(P =۰.۰۰۰۱) in tumoral tissue.Conclusion: The obtained findings suggest that DUXAP۸ acts as an oncogene in GC and maybe a new target for gene therapy of GC.

کلیدواژه ها:

Gastric cancer ، Pseudogene ، LncRNA ، DUXAP۸