Novel anticancer peptide derived of Lactoferricin-B inhibits chemo-drug metabolism in cancer cells by blocking GSTP۱ factor

سال انتشار: 1400
محل انتشار: کنفرانس بین المللی ژنتیک و ژنومیکس انسانی
کد COI اختصاصی: CHGGE01_277
زبان مقاله: انگلیسی
تعداد مشاهده: 116

نویسندگان

Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

چکیده

Backgrounds: Antimicrobial peptides (AMPs), are essential components of the immune defenseof multicellular organisms and are currently being developed as antimicrobial and antitumordrugs. Lactoferricin-B (LfcinB) is an antimicrobial peptide that has cytotoxic activity againstcancer cells. Studies have shown that Lactoferricin-B or its derivatives can inhibit tumor growthin vitro and in vivo. Thus, aim of this study is improving the approach for the identification ofpotential peptide molecule for GSTP۱ targeting through bioinformatics analysis.Materials and Methods: Lactoferricin-B sequence submits to AntiCP server for predict anddesign of newly anticancer peptides. The ۲D and ۳D structures of these peptides were drawnwith Protean (DNAstar software), Phyre۲ and I-TASSER respectively. Furthermore, anticancerproperty for each peptide expected with iACP software. The ۳D structures of peptides weredocked with the GSTP۱ using docking server HDOCK and the peptide with the maximumbinding energy value was identified.Results: AntiCP server predicts > ۳۵۰ peptides with anticancer property. Peptides with highSVM score were selected. Anticancer activity of new design peptides showed ۹۹% anticancerspecificity. Result revealed that among ۳۵۰ peptides were virtually screened ۴۵ peptides showedgreater than ۹۹% anticancer property and among these peptidesFKCRRWQWRMKKLGAPSIWCVRR identified as potential sequence for GSTP۱ ligand.Conclusion: Our results indicated that the probability of successful production of new anticancerpeptide derived of Lactoferricin-B. These outcomes based on in silico analysis may be used incancer therapy or vaccine design. Therefore, new designed peptide can be used as potentialinhibitor agent in for GSTP۱ against cancer.

کلیدواژه ها

Anticancer peptide, Lactoferricin B, Bioinformatics, GSTP۱

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.