Critical roles of extracellular matrix genes in the pathogenesis of carpal tunnel syndrome

Backgrounds: Carpal tunnel syndrome (CTS) is the most common peripheral nerve entrapmentsyndrome, affecting a large percentage of the general population. A recent study of CTSidentified a couple of CTS-associated variants within genes implicated in growth andExtracellular Matrix (ECM) architecture, although the causative link has not been established.The aim of this study is to evaluate the differential gene expression among the sample of patientssuffering from CTS and normal individuals by bioinformatics analysis.Materials and Methods: The raw data of RNA-sequencing samples of normal individuals andCTS patients were obtained from the database with the number, GSE۱۰۸۰۲۳. Then the steps ofsample analysis were performed. Genes with differential expression were isolated with DESeq۲R package. Then, important signaling pathways and the genes involved in the ECM wereinvestigated.Results: The results revealed that the expressions of ۲۳۷۴ genes were upregulated whereas theexpressions of ۲۴۷۴ genes were downregulated in patients. In addition, TNXB, ITGB۴, LAMC۳,ITGB۳, LAMA۳, TNC, DMP۱, LAMC۲, HMMR, ITGB۶, LAMB۳, ITGA۳, ITGA۲, LAMB۴, FN۱,GP۶, FRAS۱, ITGA۱, COL۴A۶, COL۴A۵, COL۶A۳, SDC۱, ITGA۵, FREM۲ genes which areupregulated or down regulated involved in the extracellular matrix receptor interaction.Conclusion: Finally, our analysis proposed the important role of extracellular matrix proteins inthe pathology of CTS. However, the accurate molecular experimental studies are needed toconfirm the role of ECM genes in the development of CTS.

کلیدواژه ها:

Carpal tunnel syndrome ، Extracellular matrix ، RNA-sequencing