Potential biomarkers and signaling pathways associated with the pathogenesis of primary salivary gland carcinoma: A bioinformatics study

Backgrounds: Salivary gland carcinoma (SGC) is rare cancer, which constitutes ۶% ofneoplasms in the head and neck area. The most responsible genes and pathways involved in thepathology of this disorder have not been fully understood. We aimed to identify differentiallyexpressed genes (DEGs), the most critical hub genes, transcription factors, signaling pathways,and biological processes (BPs) associated with the pathogenesis of primary SGC.Materials and Methods: The mRNA dataset GSE۱۵۳۲۸۳ in the Gene Expression Omnibusdatabase was re-analyzed for determining DEGs in cancer tissue of patients with primary SGCcompared to the adjacent normal tissue (adjusted P-value < ۰.۰۰۱; |log۲ Fold Change| > ۱). Aprotein interaction map (PIM) was built, and the main modules within the network wereidentified and focused on the different pathways and BP analyses. The hub genes of PIM werediscovered, and their associated gene regulatory network was built to determine the masterregulators involved in the pathogenesis of primary SGC. Furthermore, the results were validatedusing another independent dataset.Results: A total of ۱۳۷ genes were found to be differentially expressed in primary SGC. Themost significant pathways and BPs that were deregulated in the primary disease condition wereassociated with the cell cycle and fibroblast proliferation procedures. TP۵۳, EGF, FN۱,NOTCH۱, EZH۲, COL۱A۱, SPP۱, CDKN۲A, WNT۵A, PDGFRB, CCNB۱, and H۲AFX weredemonstrated to be the most critical genes linked with the primary SGC. SPIB, FOXM۱, andPOLR۲A significantly regulated all the hub genes.Conclusion: This study illustrated several hub genes and their master regulators that might beappropriate targets for the therapeutic aims of primary SGC.

کلیدواژه ها:

Biomarkers ، Gene regulatory network ، Pathogenesis ، Protein-protein interaction network ، Salivary gland carcinoma