The synergistic cytotoxic and apoptotic effect of resveratrol and Naringenin on Y۷۹ retinoblastoma cell line

Backgrounds: Resveratrol is a phenolic natural product which is found in red grapes also inJapanese knotweed root (Polygonum cuspidatum). Naringenin is one of the flavonoidcompounds found in landing grape and other citrus fruits. Both agents exert antioxidant and antiinflammatoryproperties. In this study, we investigated the effect of Resveratrol and Naringeninin an in vitro model of retinoblastoma of the eye.Materials and Methods: XTT and Trypan blue assays used to evaluate the antiproliferative/cytotixic effect of resveratrol and naringenin in Y۷۹ cells. By the aid ofAnnexinV/PI flow cytometry the kind of cell death investigated. To access important geneexpression level at mRNA level, involve in apoptosis, Real-time PCR utilized.Results: Naringenin and resveratrol significantly decreased proliferation and stimulated celldeath (mostly apoptosis) in Y۷۹ cells at ۵۰ and ۱۰۰ (μg/ml) after ۲۴ and ۴۸ hours. Morecytotoxic effect observed after ۴۸ hours. Furthermore, expression level of Bax and Bcl۲ mRNAsaltered significantly in all samples treated with ۵۰ (μg/ml) of naringenin, resveratrol, orsimultaneously with both. P۲۱ mRNAs expression altered in all mentioned samples except thosetreated with ۵۰ (μg/ml) of resveratrol.Conclusion: Based on the results, it can be concluded that resveratrol and naringenin candecrease cell viability in retinoblastoma cells in an in vitro dose/time-dependent manner. Albeitmore studies needed to shed the light on the mechanism of action, our data reveals a potentialsynergistic cytotoxic effect of naringenin and resveratrol on Y۷۹ cells in ۴۸ hours.

کلیدواژه ها:

Resveratrol ، Naringenin ، Retinoblastoma ، Synergism ، Cytotoxic ، Y۷۹ cell line