The proliferation and migration of metformin-treated vascular smooth muscle cells are suppressed via Focal Adhesion Kinase in high glucose conditions

Backgrounds: Cardiovascular diseases are known as one of the important causes of death inpatients with diabetes mellitus. Metformin is used as an oral medication for reducing bloodsugar. In this study, the effects of metformin were investigated on the FAK gene and proteinexpression levels, cell viability, and migration rate of VSMCs in high glucose conditions.Materials and Methods: The FAK gene and protein expression levels were evaluated inVSMCs treated with different values of metformin (۱ mM, ۵ mM, and ۷ mM), based on cellviability using RT-qPCR, western blotting, and MTT techniques. The cellular migration wasevaluated by scratch assay.Results: The FAK gene expression levels reduced significantly in metformin-treated VSMCs at۲۴h and ۴۸h periods (p<۰.۰۰۰۸ and p<۰.۰۰۰۱, respectively). The FAK protein expression levelsreduced significantly at ۲۴h (۵ mM and ۷ mM metformin doses) and ۴۸h (all doses) periods(p<۰.۰۰۱). In agreement with FAK protein values, cellular migration reduced significantly at ۲۴hand ۴۸h periods (p<۰.۰۰۱).Conclusion: The results showed that metformin suppresses the proliferation and migration ofVSMCs via FAK-related pathways and may retard the progression of vessel stenosis in diabetes.

کلیدواژه ها:

VSMCs ، FAK ، Metformin ، High-glucose ، Migration