Evaluation of endosomal Toll-like receptors-۳ and ۹ gene expressions in endometrial of hydrosalpinx women

Backgrounds: Infertility is the failure to achieve a pregnancy after one year of regular andunprotected sexual intercourse. Hydrosalpinx is one of the female infertilities causes whichbelong to tubular diseases. It refers to the blockage of a fallopian tube with an unusual liquid thatmaybe harm the implantation process. Reproductive health is the result of an appropriateresponse between the immune system and the reproductive system.The innate immune system is the first defensive line against microbial pathogens an enormouspart of which involves toll-like receptors. Toll-like receptors have important impacts oninfertility and implantation time. We aim to evaluate the expression of Toll-like receptors ۳ and ۹in endometrial samples of hydrosalpinx patients.Materials and Methods: Endometrial samples were obtained from the luteal phase were dividedinto case and control groups. The case group was women who had Hydrosalpinx disease (n=۱۰).The control group was infertile women with male factor causes (n=۱۰). After sampling andextraction of RNA, a syncretization of cDNAs was done. For comparison of quantitativeexpression of TLR ۳ and ۹ between two groups, QPCR was used.Results: Reduction in the expression of antiviral TLRs in the endometrium of women withHydrosalpinx compared with the control group was shown.Conclusion: As the role of the innate immune system in implantation has been identified, soalteration in the expression of one of its components, including the Toll-like receptors ۳ and ۹,can disrupt the implantation process in these patients.

کلیدواژه ها:

Hydrosalpinx ، Innate immune system ، TLR ۳ ، ۹