Computational Analysis of Notch۱ and its correlated miRNAs Involvedin Colorectal Cancer Progression

Backgrounds: The advances in computational analysis facilitated the investigation for RNAanalysis, and enabled us to study bimolecular interactions and cancer biomarkers moreefficiently due to the ability to precisely analyze large amounts of data in a short time. In cancerpathophysiology, microRNA (a small non-coding RNA that contains ۲۰-۲۵ nucleotides) hasreceived remarkable attention for its involvement in regulating the expression of a variety ofgenes. So far, several miRNAs have been found that express aberrantly in colorectal cancerwhich is one of the most prevalent occurring carcinomas worldwide. In this study, we aim tofocus on discovering the crosslink between Notch۱ and its related miRNAs as a key oncogene incolorectal carcinoma.Materials and Methods: It has been reported that the overexpression of Notch۱ is associatedwith the high proliferation of colorectal carcinoma cells; therefore, Notch۱ possesses a keyoncogenic role in colorectal cancer. The interaction between notch۱ and its target miRNA wasfound by implementing the miRTargetLink database. Overall, ۳۶ miRNA was found to be highlyassociated with notch۱ expression. Among the predicted miRNAs, four of them were nominatedfor their strong correlation with notch۱ in colorectal cancer. The candidate miRNAs were furtherexamined for KEGG enrichment pathway analysis by using Diana miRpath V.۲ algorithm.Results: The analyzed data obtained from miRTargetLink databases showed that miR-۱۸۱a-۵p,miR-۳۴a-۵p, miR-۲۴-۳p, and miR-۳۰a-۵p are significantly correlated to Notch۱. Furthermore, byusing Diana miRpath algorithm, we demonstrated that these miRNAs play important roles in cellcycle, Wnt signaling, and Hippo signaling pathways.Conclusion: Taking into account the low level of expression of miR-۱۸۱a-۵p, miR-۳۴a-۵p, miR-۲۴-۳p, and miR-۳۰a-۵p in colorectal cancer and their direct interaction with Notch۱ oncogene, itcan be observed that these miRNAs can severs as potent regulators of signaling pathwaysinvolved in colorectal cancer progression.

کلیدواژه ها:

computational analysis ، colorectal carcinoma ، microRNA ، Notcth۱