Marzieh Mehrafza - Department of Infertility and IVF, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Azadeh Raoufi - Department of Developmental Biology, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Elmira Hosseinzadeh - Department of Embryology, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Gholam Reza Pourseify - Department of Genetics, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Tahereh Zare Yousefi - Department of Obstetrics and Gynecology, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Termeh Shakery - Department of Animal Biology, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
Amirhossein Tamimi - Department of Medicine, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran

Background & Objective: Autologous platelet-rich plasma consists of concentrated autologous plasma and several cytokines and growth factors released by activated platelets in injury and inflammation. There is an increasing trend towards the effectiveness of intrauterine PRP infusion in repeated implantation failure patients. The aim of the present study was to describe the impact of intrauterine platelet-rich plasma infusion on the live birth rate in patients with repeated implantation failure. Materials & Methods: The present retrospective uncontrolled study was performed on ۹۶ patients with more than two failed intracytoplasmic sperm injection cycles at Mehr medical institute between ۲۰۱۹ and ۲۰۲۱. Forty-eight hours before embryo transfer, patients received ۱ mL lympho-platelet-rich plasma through an intrauterine insemination catheter. Patients were evaluated for pregnancy rate. Endometrial preparation for frozen-thawed embryo transfer was performed. Results: Participants' basal and stimulation characteristics, including gonadotropin dosage, the total number of oocytes, metaphase II oocytes and embryos, endometrial thickness, embryo transfer, quality of transferred embryos, and blastocyst transfer rate were evaluated. A total of ۳۳ and ۲۷ chemical (۳۴.۳%) and clinical pregnancies (۲۸.۱%) were achieved. Twenty (۲۰.۸%) and nineteen (۲۰%) cycles resulted in ongoing pregnancies or live births, respectively. Conclusion: The current study suggests that platelet-rich plasma infusion ۴۸ hours before frozen-thawed embryo transfer may be a good option for repeated implantation failure patients and results in ۲۰% live birth.

Embryo implantation, Intracytoplasmic sperm injection, Plasma enriched platelet, Pregnancy