Effect of n-۶ and n-۳ fatty acids on expression of pro-inflammatory cytokines in lambs vaccinated against foot and mouth disease virus

The current study was aim to the effects of n-۶ and n-۳ polyunsaturated fatty acids (PUFA, or palmitic acid sources) on expression of pro-inflammatory cytokines in lambs vaccinated against foot and mouth disease (FMD) virus. A total of fifteen Sangsari male lambs with an age of ۶ ± ۱ months and body weight of ۴۲ ± ۱ kg were randomly assigned to one of three experimental treatments as follows: ۱) PO: received calcium soap of palm oil fatty acid (FA) in the diet as source of palmitic acid (۱۶:۰), ۲) SO: received calcium soap of sunflower oil FAs in the diet as source of linoleic acid (n-۶ ۱۸:۲) and ۳) LO: received calcium soap of linseed oil FA in the diet as source of α-linolenic acid (n-۳ ۱۸:۳). The lambs were offered an iso-energetic and iso-nitrogenous diet for ۲۸ days (including ۲۱ days of adaptation and ۷ days of sampling). The lambs were individually housed and had access to water ad libitum. The expression of interleukin-۱β (IL-۱β) mRNA was lower in LO when compared with PO and SO (P<۰.۰۵). The expression of tumor necrosis factor-α (TNFα) mRNA was higher in PO when compared with SO and LO and the lowest expression of TNFα mRNA was measured in LO (P<۰.۰۵). Lower concentrations of serum cholesterol and triglyceride (TG) were measured in vaccinated lambs on LO diet when compared with vaccinated lambs on palmitic FA diet (P<۰.۰۵). There was no significant difference between SO and LO groups in serum cholesterol or TG levels. There were no significant differences between treatments (P>۰.۰۵) in serum level of total proteins and albumin. The findings from the current study showed feeding α-linolenic acid diet following vaccination against FMD resulted in a decrease in serum levels of cholesterol and TG due to higher n-۳ PUFA intake and suppressed the pro-inflammatory cytokine expression (IL-۱ and TNFα).  

کلیدواژه ها:

Acute-phase proteins ، Alpha-linolenic acid ، Conjugated linoleic acid ، Foot and Mouth Disease ، Linseed