New applications of PCR

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 285

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تاریخ نمایه سازی: 22 شهریور 1401

چکیده مقاله:

PCR is a highly advanced, but at the same time a simple technique whoseversatility and application is proved due to its ability to provide qualitativeand quantitative results in most biological and clinical fields. The inventionof PCR along with its application in clinical diagnostics, DNA profiling,recombinant DNA and other fields such as anthropology, crime, etc. hashad a tremendous impact on the process of molecular science. Sequencingthe human genome and the genome of other organisms has enabled PCR tobe useable in a variety of ways to accurately analyze the data. Thesewidespread applications are the result of a change in the basic PCRtechnique resulted in other forms such as RT-PCR, qPCR, Multiplex PCR,Nested PCR, Miniprimer PCR. Today, new generations of PCR, includingExtreme PCR, Photonic PCR, ColdPCR and Nanoparticle PCR, haveemerged minimizing the limitations of long duration and nonspecificreplication, etc.

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Seyed Hossein Khaleghinejad

Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences,Shiraz University, Shiraz, Iran

Narges-alsadat Afzalian Boroujeni

Department of Biology, College of Sciences, Shahrekord University, Shahrekord, Iran