Allium cepa Inhibits Aspergillus fumigatus Growth and Virulence and Suppresses the Expression of glip Gene Involved in Fungal Pathogenesis
- سال انتشار: 1401
- محل انتشار: فصلنامه عفونت، اپیدمیولوژی و پزشکی، دوره: 8، شماره: 2
- کد COI اختصاصی: JR_IEM-8-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 265
نویسندگان
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran ۱۴۱۱۵-۳۳۱, Iran
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran ۱۴۱۱۵-۳۳۱, Iran
Department of Mycology, Pasteur Institute of Iran, Tehran ۱۳۱۶۴, Iran
چکیده
Backgrounds: Allium cepa L. as a traditional medicine is a rich source of beneficial bioactive metabolites. In the present study, the effect of A. cepa ethanolic extract (EAC) was studied on Aspergillus fumigatus growth, ergosterol synthesis, gliotoxin production, and gliP gene expression. Materials & Methods: The minimum inhibitory concentration (MIC) of EAC (۱۲۵-۴۰۰۰ µg/mL) was determined against A. fumigatus isolates according to Clinical and Laboratory Standards Institute (CLSI) guidelines (M-۳۸). Protease activity, gliotoxin production, cell membrane ergosterol content, ultrastructure, and gliP gene expression were evaluated in the fungus exposed to ۰.۵× MIC concentrations of EAC (۱۰۰۰ μg/mL) and fluconazole (FCZ: ۶۴ μg/mL). Findings: Ergosterol content was significantly reduced to ۰.۵۳ and ۰.۴۵ µg/mg in FCZ- and EAC-treated fungal cells, respectively (p< .۰۰۱). The protease activity was significantly inhibited in both EAC- and FCZ-treated groups. The gliotoxin production was inhibited by ۵۱.۵۵ and ۶۸.۷۵% in the treated groups with FCZ and EAC, respectively. The expression of gliP in both EAC- and FCZ-treated A. fumigatus groups was significantly reduced by ۰.۴۰ and ۰.۵۳-fold, respectively (p< .۰۵). Conclusion: This study finding revealed that A. cepa ethanolic extract (EAC) effectively suppressed the growth and virulence factors of A. fumigatus, which could be attributed in part to its bioactive metabolites. Further studies are recommended to isolate and identify these metabolites as potential candidates for the development of antifungal drugs. < div class="msocomtxt" id="_com_۱" language="JavaScript" onmouseout="msoCommentHide('_com_۱')" onmouseover="msoCommentShow('_anchor_۱','_com_۱')">کلیدواژه ها
Aspergillus fumigatus, Allium cepa, Antifungal activity, Ergosterol, Gliotoxin, gliPاطلاعات بیشتر در مورد COI
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