Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity
- سال انتشار: 1401
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 9
- کد COI اختصاصی: JR_IJBMS-25-9_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 171
نویسندگان
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Chemical Injuries Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
چکیده
Objective(s): The high resistance rate of Acinetobacter baumannii and the limited number of available antibiotics have prompted a worldwide effort to develop effective antimicrobial agents. Accordingly, identifying single-chain variable fragment antibodies (scFvs), capable of exerting direct antibacterial activity in an immune system-independent manner, may be making immunocompromised patients more susceptible to A. baumannii infections.Materials and Methods: To isolate bactericidal scFvs targeting A. baumannii, we panned a large human scFv phage display library against whole-cell extensively drug-resistant (XDR) A. baumannii strains grown as biofilm or cultured with human blood or human peripheral blood mononuclear cells plus plasma. The binding of scFv-phages to A. baumannii was assessed by the dot-blot assay. Soluble scFvs, derived from the selected phages, were assessed based on their ability to bind and inhibit the growth of A. baumannii. Results: Five phage clones showed the highest reactivity toward A. baumannii. Among five soluble scFvs, derived from positive phage clones, two scFvs, EB۲۱۱ and EB۲۷۹, had high expression yields and displayed strong binding to A. baumannii compared with the controls. Moreover, XDR A. baumannii strains treated with positively-charged scFvs, including EB۲۱۱, EB۲۷۹, or a cocktail of EB۲۱۱ and EB۲۷۹ (۲۰۰ µg/ml), displayed lower viability (approximately ۵۰%, ۷۸%, and ۴۰% viability, respectively) compared with PBS-treated bacteria.Conclusion: These results suggest that combining last-resort antibiotics with bactericidal scFvs could provide promising outcomes in immunocompromised individuals with A. baumannii infections.کلیدواژه ها
Acinetobacter baumannii, Antibacterial agents, Colistin, Monoclonal antibody, Phage display library, Single-chain variable-fragmentاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.