Construction and Stability of Glucose Biosensor Based on Carbon Past Electrode Modified with Colloidal Gold Nanoparticles

Using glucose biosensor is important in detection various amounts of glucose. In this paper, a biosensor electrode called (Aunano/CPE) electrode was prepared which is a modified carbon fermentation electrode with colloidal gold nanoparticles composed of carbon graphite powder, paraffin oil and colloidal gold nanoparticles (24nm dia.) and put in contact with glucose oxidase enzyme in the presence of phosphate buffer by a semi-permeable membrane. Ag/AgCl reference electrode and (Aunano/CPE) electrode were used for performing biosensor amperometric and glucose was detected in a definite concentration range by applying 0.7V constant potential to the electrodes. Experiment design was performed by Taguchi method for both glucose concentration and pH factors on three levels for which the optimum values were obtained as 1mM and 6, respectively. The biosensor remained stable for 6 days based on the activity of glucose oxidase enzyme in optimum conditions

کلیدواژه ها:

biosensor ، electrode ، glucose ، amperometric ، glucose oxidase