Investigating the Association of rs۱۶۱۷۶۴۰ Polymorphism in Patients with Diabetes Type II and Its Complications

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 174

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شناسه ملی سند علمی:

JR_JHGG-1-1_005

تاریخ نمایه سازی: 30 مرداد 1401

چکیده مقاله:

The prevalence of diabetes – especially diabetes type II- is increasing steadily; according to WHO reports it will increase to ۳۶۶ millionpeople by the year ۲۰۳۰. Microvascular complications including Proliferative diabetic retinopathy (PDR) and end stage renaldisease (ESRD) are increased in patients with diabetes mellitus. Case-control association studies have demonstrated that rs۱۶۱۷۶۴۰SNP in the promoter of erythropoietin (EPO) gene is significantly associated with PDR and ESRD. In the mentioned SNP, TT genotypeis considered as risk genotype which means that EPO concentration in human vitreous body in these people shall be higher.People with TT genotype are much more at risk of retinopathies. In this study we investigated the existence of rs۱۶۱۷۶۴۰ EPO genepolymorphism among ۱۵۰ healthy subjects and ۱۵۰ subjects with diabetes type II who referred to Yazd central laboratory. Then theassociation of rs۱۶۱۷۶۴۰ SNP with complications among diabetic patients were examined by ARMS-PCR method. The results wereanalyzed using GraphPad software (version ۵.۰۰). Prevalence of genotype GG was ۸% in patients and ۱.۳% in the control group. GTwas ۵۱.۳% in patients, and ۸۶.۷% in the control group, and finally TT was observed in ۴۰.۷% in patients, and ۱۲% of control group. TheTT genotype was ۳۷.۶% in patients with retinopathy and ۴۲.۶% in non-retinopathy patients. Our study demonstrates that the prevalenceof rs۱۶۱۷۶۴۰ SNP has significant difference between diabetic patients and control group; whereas there was not any significantrelationship between this polymorphism and the complications of diabetes in patients. Together our study reveals that rs۱۶۱۷۶۴۰SNP may be associated with susceptibility to diabetes type II; however it seems that this polymorphism is not significantly relatedto the diabetic complications in Yazd.

نویسندگان

Mehdi Roshanian

Genetics Biology, Ashkezar Branch, Islamic Azad University, Ashkezar, Iran

Mohammad Amin Javidi

Department of Molecular and Cellular Science, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

Negin Jafarian

Genetics Biology, Ashkezar Branch, Islamic Azad University, Ashkezar, Iran

Mohammad Hasan Sheikhha

Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran