The Effect of Fasting on Oxidative Stress in the Vital Organs of New Zealand White Rabbit
- سال انتشار: 1401
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 11، شماره: 2
- کد COI اختصاصی: JR_RBMB-11-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 123
نویسندگان
Department of Biochemistry & Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia & Center of Hypoxia & Oxidative Stress Studies, Department of Biochemistry & Molecular. Biology, Faculty of Medicine Universitas Indonesia, Jaka
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
چکیده
Background: Oxidative stress is defined as the condition in which balance between the synthesis and detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand White (NZW) rabbits. Methods: Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting (PF) groups. The controls were fed ad lib. IF and PF groups were fasted for ۱۶ and ۴۰ hours, respectively, followed by eight hours of non-fasting, for six days and were sacrificed on the ۷th day. One hundred mg of heart, brain, and kidney tissues were homogenized in ۱ ml of phosphate-buffered saline. MDA, carbonyl, GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and post hoc test. Results: In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was significantly less in the PF than in the control group. Conclusions: The effect of fasting on oxidative stress in various organs showed various responses, however fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.کلیدواژه ها
Fasting, Oxidative stress, Vital organs, Rabbitاطلاعات بیشتر در مورد COI
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