Effects of wasp venom on venous thrombosis in rats

  • سال انتشار: 1401
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 7
  • کد COI اختصاصی: JR_IJBMS-25-7_005
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 227
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نویسندگان

Fan-mao Jin

Lishui City People’s Hospital, Lishui, Zhejiang ۳۲۳۰۰۰, People’s Republic of China

Mei Wang

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

Xiumei Wu

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

Huai Xiao

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

Dexiao Wang

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

Guang-ming Wang

Genetic Testing Center, The First Affiliated Hospital of Dali University, Dali University, Dali, China

Cheng-gui Zhang

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

Hai-rong Zhao

Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China

چکیده

Objective(s): This study aimed to investigate the potential effects of wasp venom (WV) from Vespa magnifica on antithrombosis in rats with inferior vena cava (IVC) thrombosis.Materials and Methods: The thrombosis rat model was established by improving the IVC stenosis, in which rats were subjected to IVC ligation for ۷۵ min. Rats were administered argatroban (IP) or WV (s.c.) for ۴ hr after IVC thrombosis. The weight, inhibition rate, and pathological morphology of the thrombosis induced by IVC ligation and the variation in four coagulation parameters, coagulation factors, and CD۶۱+CD۶۲P+ were simultaneously determined in IVC rats.Results: The thrombus formed as a result of IVC ligation was stable. Compared with the control group, the weight of the thrombus was significantly reduced in the argatroban group. Thrombus weight was reduced by treatment with ۰.۶, ۰.۲, and ۰.۰۵ mg/kg WV, with inhibition rates of ۵۲.۱۹%, ۳۵.۳۲%, and ۲۸.۹۸%, respectively. Inflammatory cells adhered to and infiltrated the vessel wall in the IVC group more than in the sham group. However, the pathological morphology and CD۶۱+CD۶۲P+ of the WV treatment groups tended to be normal. Conclusion: We improved the model of IVC thrombosis to be suitable for evaluation of antithrombotic drugs. Our findings demonstrated that WV could inhibit IVC thrombosis associated with reducing coagulation factors V and CD۶۱+CD۶۲p expression in rats.

کلیدواژه ها

Argatroban, Blood coagulation factors, Platelet activation, Venous thrombosis, WV

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