Haniyeh Mahmoudinia - Department of Biology, Payame Noor University (PNU), P.O. Box, ۱۹۳۹۵- ۳۶۹۷, Tehran, Iran
Sattar Tahmasebi Enferadi - Department of Molecular Plant Biotechnology, Faculty of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Zahra Vaezi Sooghanloo - Department of Biology, Payame Noor University (PNU), P.O. Box, ۱۹۳۹۵-۳۶۹۷, Tehran, Iran
Bahareh Azimi Sarikhan Begloo - Department of Biology, Payame Noor University (PNU), P.O. Box, ۱۹۳۹۵-۳۶۹۷, Tehran, Iran
Forouzandeh Mahjoubi - Department of Molecular Plant Biotechnology, Faculty of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Peganum harmala L is a perennial herbaceous plant that is very important in the herbal medicine industry. Which is used in medicine and health (۱) In this study, using FTIR as a method to determine the functional groups of the active ingredients of Peganum harmala L has been investigated.In this study, ۲۰ g of Pecan seed was well stirred for ۱۰ minutes after grinding in ۳۰% (v / v) acetic acid solution. The mixture was passed through ۰.۴۵ nm filter paper. And washed again with acetic acid. And petroleum ether and ethyl acetate were added ۱۰۰ ml each. Then it was brought to pH = ۸. Finally, chloroform was added to the above solution.The obtained alkaloids were collected in the chloroform phase after