A Chahardoli - Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran

Pseudomonas aeruginosa is a highly resistant gram-negative bacterium that causes many diseases, infections and, food spoilage. Therefore, research onnew antibacterial agents with the biocompatibility and ability to eliminatepathogens and limit biofilm formation is essential. Nowadays, metallicnanoparticles (NPs) such as gold NPs (AuNPs) are recognized as novelantimicrobial agents due to their high surface area to volume ratio andunique physico-chemical properties. AuNPs are widely used in biologicaland biotechnological applications as biocidal agents. In the present study,green synthesized gold nanoparticles using wild fennel; Nigella arvensis leafaqueous extract (F-AuNPs) as a potent medicinal plant with excellentproperties were applied as a novel antibacterial agent against a multidrugresistant(MDR) P. aeruginosa as a gram-negative strain. The results of thisstudy showed good antibacterial effects of F-AuNPs with minimal inhibitoryconcentration (MIC) of ۶۲. ۵ μg/mL and the inhibition zone of ۱۰ mm againstMDR P. aeruginosa. These results introduced the F-AuNPs as a newantibacterial agent for application in various fields, particularly in the foodindustry

Antibacterial; AuNPs; Biosynthesis; Pseudomonas aeruginosa; Wild fennel.