The cytotoxicity effect of biologically synthesized bismuth oxide nanoparticles using Vibrio sp. VLC on gastric cancer cells

Biosynthesis of nanoparticles (NPs) is an eco-friendly, safe and cost-effective approach, specifically when bacteria are used to produce NPs. In addition, loading of NPs on minerals such as zeolite affects their diffusion in various solutions. Gastric cancer ranks as ۵th for incidence and ۴th for mortality among all cancer types globally. To introduce novel NPs with toxic effects on gastric cancer cells, we synthesized bismuth oxide (Bi۲O۳) NPs, alone and loaded on zeolite (Bi۲O۳-Z) using a bacterium Vibrio sp. VLC, and evaluated their cytotoxicity on MKN-۴۵ cells. In this regard, Bi۲O۳ NPs (in two forms of heated and non-heated) and Bi۲O۳-Z nanocomposites (NCs, with ۳.۳۵ and ۱۵.۵۶ wt%) were synthesized, while bismuth salt and zeolite were considered as controls. For treatment of cells, ۲۵, ۵۰ and ۱۰۰ ʽg/ml of all agents were freshly prepared and after ۲۴ h, alamar Blue assay was carried out to determine cell viability. Results of this study revealed that heated and non-heated Bi۲O۳ NPs induced their cytotoxic effects in a dose-dependent manner. In addition, toxicity of Bi۲O۳-Z NCs (۳.۳۵ wt%) was more than Bi۲O۳-Z NCs (۱۵.۵۶ wt%), bismuth salt and zeolite. In conclusion, obtained findings indicated that toxicity of bismuth salt increased when formed as NPs and NCs. Nevertheless, more research is necessary to determine the mechanism of observed actions

کلیدواژه ها:

Bismuth oxide nanoparticles ، gastric cancer ، cytotoxicity ، Vibrio sp. VLC.