Introduction of new derivatives of Biotin and DTPA for labeling of antibodies with ¹¹¹In to detect malignant tumors [Persian]
محل انتشار: مجله پزشکی هسته ای ایران، دوره: 10، شماره: 2
سال انتشار: 1381
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 115
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IRJNM-10-2_008
تاریخ نمایه سازی: 6 تیر 1401
چکیده مقاله:
Radiolabeled monoclonal antibodies, have created new innovations in diagnosis, research and therapy of disease in last ۲ decades. One of the serious limitation of applications of radiolabeled antibodies in vivo is relatively low target to background activity. Various strategies have been proposed to solve this problem including pre-targeting methods that was suggested in ۱۹۸۹. Regarding importance of monoclonal antibodies and radioisotopes, based on pre-targeting strategy, we have introduced new derivative of Biotin and DTPA to decrease background activity. DTPA-bio and new derivative (DTPA-bio-۱OX) were labeled with ¹¹¹In, labeled compounds and injected through tail veins into Balb/c mice, and percent of injected dose per gram of blood (%ID/g of blood) was determined at ۱۵, ۳۰, ۶۰, ۱۲۰, ۱۸۰, and ۲۴۰ min after injection. Based on results,¹¹¹In-DTPA-bio rapidly cleared from serum, indicating molecular weight of label is increased causing delayed clearance from serum. Therefore, there is enough time for label to accumulate in the target tissues. With advent of second generation of monoclonal antibodies and antibody engineering, pretargeting methods have changed greatly. It seems that derivatives we introduced will have an important role in new pre-targeting methods.
کلیدواژه ها:
Monoclonal antibody (MAB) ، Diethylentriaminepantaaceticacide (DTPA) ، Indium-۱۱۱ (¹¹¹In) ، Fast performance liquid chromatography (FPLC)
نویسندگان
Soraya Shahhosseini
Faculty of Pharmacy, Shaeed Beheshti University of Medical Sciences, Tehran, Iran
Gholamreza Farshidfar
Faculty of Medicine, Bandarabas University of Medical Sciences, Bandarabas , Iran
Reza Najafi
Department of Radioisotope, Nuclear Research Center, AOEI, Tehran, Iran