A Significant Reduction in the Plasma Levels and Gene Expression of CCL۲ in Patients with Osteoarthritis following Intervention with Krocina™
- سال انتشار: 1401
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 11، شماره: 1
- کد COI اختصاصی: JR_RBMB-11-1_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 164
نویسندگان
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran & Immunology Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran & Internal Medicine Department, Imam Reza hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Immunology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
School of Medicine, Bam University of Medical Sciences, Bam, Iran.
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده
Background: inflammatory chemokines such as CCL۲ and CCL۵ are involved in the progress of osteoarthritis. Crocin with antioxidant and anti-inflammatory properties can reduce the symptoms of osteoarthritis (OA). This study was performed investigate the effect of Krocina™, on the gene expressions and plasma levels of CCL۲ and CCL۵ in OA patients. Methods: The study included ۳۵ patients that were randomized in the KrocinaTM and placebo groups. The intervention was Krocina™ ۱۵ mg daily for four months. Clinical and paraclinical parameters were measured. CCL۲ and CCL۵ genes expression and plasma levels were determined using the SYBR Green Real-Time RT-PCR and Enzyme-linked Immunosorbent Assay (ELISA) techniques. Results: The C-reactive protein (CRP) value in the KrocinaTM group and the visual analogue scale (VAS) value in the KrocinaTM and placebo groups decreased significantly after the intervention. The gene expression of CCL۲ in the KrocinaTM and placebo groups decreased significantly. On the contrary, the gene expression of CCL۵ in the KrocinaTM and placebo groups increased significantly. Moreover, the plasma levels of CCL۲ in the KrocinaTM and placebo groups decreased meaningfully. There was no difference regarding the plasma levels of CCL۵ within the Krocina™ and placebo groups before and after the intervention in either of the groups. Conclusions: Administration of Krocina™ reduced the clinical signs of inflammation and CRP and VAS value. Also, Krocina™ significantly decreased the plasma levels and gene expression of CCL۲ in osteoarthritis patients.کلیدواژه ها
CCL۲, CCL۵, Krocina™, Osteoarthritis.اطلاعات بیشتر در مورد COI
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