Masoumehalsadat Rahmati - Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Samira Shokri - Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mehdi Ahmadi - Department of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mehdi Ahmadi - Department of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Narges Marvi Moghadam - Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Saeide seyfi - Department of Physical Education, Yadegar-e-Imam Khomeini (RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran
Melika Goodarzi - Kahrizak Kavosh Research Institute, Tehran
Reza Hazrati-Raziabad - Department of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

According to the importance of Anopheles mosquitoes, in the transmission of malaria and control of disease transmitter is one of the important strategies for the prevention of diseases. This study aimed to produce plant insecticides using blue extracts of oleander plants, tobacco, ferula, and Achilles Mille folium essential oils and copper oxide nanoparticles synthesized by pomegranate peel extract on the Anopheles Stephens mosquitoes to solve the problems by chemical insecticides. In this laboratory research, the effect of blue extracts with concentrations (۰.۶۶, ۱.۳, and ۲.۶) microliters in a milliliter of leaves and stems of oleander, tobacco, ferula, and Achilles Mille folium essential oil on the Anopheles mosquito (Anopheles. Stephens: Bandar Abbas strain) was studied. To optimize these extracts, antibacterial properties of thyme were used to prevent mold growth. In addition, the insecticidal effect of copper oxide nanoparticles synthesized from pomegranate peel extract was considered. To identify nanoparticles synthesized by spectroscopic analysis Vis-UV, scanning electron microscopy (SEM) and X-ray energy diffraction (EDX) was used. The results showed that copper oxide nanoparticles of pomegranate extract with antibacterial properties were obtained with LC۵۰ = ۰.۷۰. It had a good insecticidal effect on Anopheles Stephens mosquito specimens. But oleander plants with LC۵۰ less than copper Nan oxide showed more lethality.  

Copper Oxide Nanoparticles, Anopheles Stephens mosquito, SEM, Oleander, Tobacco, Pomegranate