Fabrication and characterization of PCL/Keratin nanofiber wound dressing containing PRF: in vitro and in vivo study

سال انتشار: 1401
محل انتشار: ششمین کنفرانس بین المللی پژوهش های کاربردی در علوم و مهندسی
کد COI اختصاصی: CARSE06_050
زبان مقاله: انگلیسی
تعداد مشاهده: 363

نویسندگان

Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran

Sheyda Labbaf

Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

Mohamadreza Tavakoli

Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

Vida haghighi

Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

parvin ahmaditabbar

Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University,Tehran, Iran

چکیده

Electrospun nanofibers represent a novel class of materials that show great potential in many biomedical applications including biosensing, regenerative medicine, tissue engineering, drug delivery and wound healing. The aim of this study was to evaluate the wound healing process using polycaprolactone (PCL)/ kreatine (Kr)/ platelet-rich fibrin (PRF) scaffold Prepared by electrospinning method and in vitro and in vivo examinations were done. The morphology of nanofiber dressings was investigated using scanning electron microscopy (SEM) and functional groups and chemical bonds by Fourier transform infrared spectroscopy (FTIR). Then contact angle and degradability were investigated. With the addition of PRF to the PCL / Kr scaffold, the fiber diameter decreased from nm ۱۴۰/ ۰۱± ۳۴ nm to ۶۵/۹۸±۱۴. Biocompatibility was assessed using MTT assay with L۹۹۹ cells and the results showed that PRF-containing dressings improved cell viability. The results of this study show that the scaffold prepared from PCL / Kr / PRF is a suitable candidate for use as a wound dressing as well as for use in skin tissue engineering applications.

کلیدواژه ها

Keratin, Nanofibers, Platelet-rich fibrin, Polycaprolactone, Wound dressing.

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