Neutrophil depletion reduces interstitial cajal-like cell injury and alleviates inflammation-induced motor dysfunction in guinea-pig gallbladder during acute cholecystitis
- سال انتشار: 1401
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 4
- کد COI اختصاصی: JR_IJBMS-25-4_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 303
نویسندگان
Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, No. ۱, Youyi Road, Yuzhong District, Chongqing ۴۰۰۰۱۶, China
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. ۲۳۸, Jiefang Road, Wuhan ۴۳۰۰۶۰, Hubei Province, China
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. ۲۳۸, Jiefang Road, Wuhan ۴۳۰۰۶۰, Hubei Province, China
Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, No. ۱, Youyi Road, Yuzhong District, Chongqing ۴۰۰۰۱۶, China
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. ۲۳۸, Jiefang Road, Wuhan ۴۳۰۰۶۰, Hubei Province, China
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, No. ۱, Youyi Road, Yuzhong District, Chongqing ۴۰۰۰۱۶, China
چکیده
Objective(s): Gallbladder interstitial Cajal-like cells (ICLCs) are known as some of the players in the complex motility mechanisms affecting gallbladder motility. This study aims to explore the mechanism of guinea-pig gallbladder motility disorders during Acute Cholecystitis (AC), focusing on the relationships between neutrophil alterations, gallbladder ICLCs, and smooth muscle contractility.Materials and Methods: Forty-eight guinea pigs were randomly divided into four groups: normal, sham, common bile duct ligation (CBDL), and anti-PMN (anti-polymorphonuclear antibody treated +CBDL). Hematoxylin and eosin-stained slides from each gallbladder sample were examined for inflammation, and myeloperoxidase (MPO) activity was evaluated. The contractile response of gallbladder muscle to Ach, CCK-۸, and KCl was registered by a tension transducer, and ultrastructure features of ICLCs were observed.Results: Pretreatment with anti-PMN significantly reduced the circulating neutrophils by ۸۰% and also considerably decreased the gallbladder MPO activity by ۵۲.۹% compared with the CBDL group (P< ۰.۰۵). After adding Ach, CCK-۸, and KCl, the contraction ability in CBDL and anti-PMN groups was lower than those of normal and sham groups (P< ۰.۰۵), and they were increased substantially in the anti-PMN group compared with the CBDL group (P< ۰.۰۵). Transmission electron microscopy confirmed that the cytoplasm of the neutrophils was full of granules, and neutrophils contacted closely with ICLCs. The ultrastructure of ICLCs in the anti-PMN group was less inflamed and the endoplasmic reticulum was mildly dilated, and cell processes also increased.Conclusion: Anti-PMN could relieve the ultrastructure injury of ICLCs and alleviate gallbladder dysmotility during AC. Neutrophils may damage gallbladder ICLCs at first followed by dysmotility.کلیدواژه ها
Common bile duct ligation, Gallbladder dysmotility, Guinea pigs, Interstitial cajal-like cells, Neutrophils, Ultrastructure featuresاطلاعات بیشتر در مورد COI
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