Therapeutic effect of recombinant adenovirus on AGS cancer cell line
- سال انتشار: 1400
- محل انتشار: مجله Medbiotech، دوره: 5، شماره: 4
- کد COI اختصاصی: JR_MBT-5-4_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 341
نویسندگان
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University of Tehran North Branch, Tehran, IR Iran.
Cellular and Molecular Research Center, Faculty of Basic Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University of Tehran North Branch, Tehran, IR Iran.
Cellular and Molecular Research Center, Faculty of Basic Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran.
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University of Tehran North Branch, Tehran, IR Iran.
Cellular and Molecular Research Center, Faculty of Basic Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran.
چکیده
Oncolytic viruses are tumor selective and potential anticancer agents. Adenovirus type ۵ is the most commonly used oncolytic agent for treating tumors. The poor expression of the viral receptor on the surface of tumor cells is a major problem in the use of adenovirus type ۵. The study aimed to improve the attachment of the modified adenovirus to tumor cells by applying an analog of somatostatin [SST] [i.e., octreotate]. In the adenovirus fiber knob's HI-loop, the octreotate sequence was introduced. By utilizing the calcium phosphate technique, modified virus was transmitted to HEK ۲۹۳ packing cells, and a new virus was generated. Using the TCID۵۰ technique, the virus titer was determined. The human adenocarcinoma stomach cell line was challenged with modified adenovirus and wild-type ۵ strain [AGS]. Next, compounds were investigated for cytopathic impacts. The modified virus was validated employing PCR and sequencing. In comparison to the wild-type adenovirus, the modified adenovirus was found to be more capable of infecting AGS cells. Experiments showed that over ۸۱% of AGS cells treated with fiber-modified Ad۵ were killed after three to four days. This study suggests that fiber-modified Ad۵ is an oncolytic agent against AGS cells.کلیدواژه ها
Adenovirus, Virotherapy, Octreotate, AGSاطلاعات بیشتر در مورد COI
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