Experimental investigation of gene expression changes under the discontinuous electromagnetic fields in Gastric Cancer

Gastric cancer is one of the most prevalent and deadliest cancers in the world. Environmental factors, including chemicals, sunlight, and electromagnetic fields can induce changes in gene expression. Although the resizing mechanism of its effect has not been fully recognized, free radicals are seen as the possible mechanism involved. In this study the AGS cell line was cultured and exposed to electromagnetic fields discontinuously with densities of ۰.۲ and ۲ mT. Moreover, Cell viability was assessed. The change in the expression of genes is measured by real-time PCR. Results show that low frequency-electromagnetic fields increased gene expression compared to the control group. The changes in the expression are directly associated with the electromagnetic field strength. For example, SOCS۳ was significantly up regulated under the exposure of discontinuous magnetic flux with density of ۲mT.In general, all experimental groups under the illuminated fields have increased in expression. This is directly associated with the field-strength increase, with more pronounced changes in expression in the group subjected to intermittent radiation

کلیدواژه ها:

Gastric Cancer cell line ، CTSL۲ ، SOCS۳ ، Discontinuous Electromagnetic fields