Preconditioning Adipose-derived Stem Cells with Photobiomodulation Significantly Increased Bone Healing in a Critical Size Femoral Defect in Rats

  • سال انتشار: 1400
  • محل انتشار: اولین کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی، داروسازی وصنایع غذایی
  • کد COI اختصاصی: SBPFST01_014
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 300
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نویسندگان

Armin Khosravipour

A. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abdollah Amini

B. Department of Anatomy, Faculty of Medicine, Tehran Medical sciences , Islamic Azad university ,Tehran, Iran

Fatemeh Zare

C. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC, Louisville, Kentucky; USA.

Ava parvandi

Mehrdad Asgari

Sufan Asgari

چکیده

We assessed the combined impacts of human demineralized bone matrix (hDBM) scaffold, adipose-derived stem cells (hADS), and photobiomodulation (PBM) on bone repair of a critical size femoral defect (CSFD) in ۷۲ rats. The rats were divided into six groups: control (group ۱); ADS (group ۲ - ADS transplanted into hDBM); PBM (group ۳ - PBM-treated CSFDs); ADS+PBM in vivo (group ۴ - ADS transplanted into hDBM and the CSFDs were treated with PBM in vivo); ADS+PBM in vitro (group ۵ - ADS were treated with PBM in vitro, then seeded into hDBM); and ADS+PBM in vitro+in vivo (group ۶ - PBM-treated ADS were seeded into hDBM, and the CSFDs were treated with PBM in vivo. At the anabolic phase (۲ weeks after surgery), bone strength parameters of the groups ۵, ۶, and ۴ were statistically greater than the control, ADS, and PBM in vivo groups (all, p=۰.۰۰۰). Computed tomography (CT) scans during the catabolic phase (۶ weeks after surgery) of bone healing revealed that the Hounsfield unit (HU) of CSFD in the groups ۲ (p=۰.۰۰۰) and ۵ (p=۰.۰۱۹) groups were statistically greater than the control group. The groups ۵, ۴, and ۶ had significantly increased bone strength parameters compared with the PBM in vivo, control, and ADS groups (all, p=۰.۰۰۰). The group ۵ was statistically better than the groups ۴, and ۶ (both, p=۰.۰۰۰). In vitro preconditioned of hADS with PBM significantly increased bone repair in a rat model of CSFD in vivo

کلیدواژه ها

Critical size bone defect, Fracture healing, Demineralized bone scaffold, Human adipose-derived stem cell, Photobiomodulation

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