Prevalence Escherichia coli, Klebsiella and Enterobacter Species and AmpC-producing Enterobacteriaceae in Clinical Specimens of Hospitals Affiliated to Babol University of Medical Sciences, Iran using Phenotypic and Molecular Methods
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 121
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شناسه ملی سند علمی:
JR_IJMM-16-3_004
تاریخ نمایه سازی: 28 فروردین 1401
چکیده مقاله:
Background and Objective: AmpC-producing bacteria are a severe threat to treating infectious diseases caused by gram-negative bacteria. The actual prevalence of these bacteria is not clearly determined as there is no reliable diagnostic method available to detect them. Therefore, this study was performed to determine the frequency of Escherichia coli, Klebsiella, and Enterobacter species producing AmpC among clinical samples by phenotypic and molecular methods.
Methods: In this study, ۱۶۳ bacteria of Enterobacteriaceae species isolated from different clinical samples in ۲۰۱۸ were examined. Suspected isolates of producing pAmpC were identified using cefoxitin disk (FOX) and disk diffusion method. Three confirmatory phenotypic methods were performed to identify pAmpC production, and blaDHA, blaFOX, blaMOX genes were searched using a molecular method for all bacteria. Specificity and sensitivity of phenotypic tests were obtained compared to the presence of blaDHAgene.
Results: Of ۱۶۳ bacteria, ۸۰ (۴۹.۱%) isolates were resistant to FOX, and ۲۱ (۱۲.۹%) carried the blaDHA gene. Among the bacteria carrying the gene, ۵ (۶%) isolates were sensitive to FOX. ۴۹ (۶۱.۳%) FOX-resistance bacteria were positive in one of the chromosomal and/or plasmid phenotypic tests. The highest specificity and sensitivity were observed in the AmpC disk (۹۰.۸%) and CAM (۴۲.۷%) methods, respectively.
Conclusion: It seems phenotypic methods are more successful in distinguishing true negatives (higher specificity). Also, sensitivity to cefoxitin is not a criterion for not producing the enzyme AmpC. For this reason, it is recommended that national monitoring be performed to identify the genes of AmpC producing bacteria.
کلیدواژه ها:
AmpC ، blaDHA ، Enterobacter ، Escherichia coli ، Klebsiella ، PCR ، بتالاکتام ، اشریشیا کلی ، PCR ، AmpC ، blaDHA
نویسندگان
Zahra Shahandeh
Department of Laboratory Sciences, Faculty of Paramedical Sciences, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Farahnaz Sadighian
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Narges Kalantrai
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
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