Improvement of Cardiac Function Following Men-strual Blood Stem Cells Administration by Prevention of Cartilaginous and Osseous Development in Rat Model ofMyocardial Infarction
- سال انتشار: 1399
- محل انتشار: بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
- کد COI اختصاصی: RROYAN21_037
- زبان مقاله: انگلیسی
- تعداد مشاهده: 266
نویسندگان
Faculty of Veterinary Medicine, Shahrekord University, sharekord, Iran
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Nanobiotechnology Research Center, Avicenna Research Institute,ACECR, Tehran, Iran
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
چکیده
Objective: Menstrual blood stem cells (MenSCs) are intro-duced as cells with high therapeutic potential in regenerative medicine. To prove the efficiency of MenSCs administration in myocardial infarction (MI) we assessed the effects of intramyo-cardial injection of MenSCs accompanied by their derived con-ditioned medium (CM) on cardiac function in MI rat model.Materials and Methods: All animals received human care ac-cording to the criteria outlined in declaration of Helsinki. The study was approved by bioethics committee of Avicenna Re-search Institute. ۴۰ rats were randomly divided into four groups including sham, MI (PBS), CM, and MenSCs + CM. Echocar-diography and histopathological evaluation were performed at day ۷ and ۲۸ days post-MI.Results: The results demonstrated significant improvement of cardiac function in terms of ejection fraction (EF) and frac-tional shortening (FS) after MenSCs + CM injection at day ۷ (P< ۰.۰۵) and day ۲۸ (P< ۰.۰۰۱) compared to MI group. In ad-dition, transplantation of MenSCs + CM resulted in higher FS and EF compared to CM-received group (P< ۰.۰۵ and P< ۰.۰۵ respectively) at day ۲۸. Histological and immunohistochemical analyses indicated that administration of MenSCs + CM led to the survival of cardiomyocytes, modulate inflammatory phase, promote neo-angiogenesis, prevent the formation of metaplas-tic changes and mitochondrial transfer from MenSCs to cardio-myocytes and non-cardiomyocytes. Moreover, MenSCs evoked inhibition of nuclear factor-κB (NF-κB) expression, bone mor-phogenetic protein-۲ (BMP-۲) up-regulation and amplifying osteocalcin signaling in preserved cardiomyocytes.Conclusion: MenSCs accompanied by CM ameliorated the function of cardiac, prevented metaplastic development, and spurred cardiomyocyte survival following MI.کلیدواژه ها
Myocardial Infarction, Menstrual Blood Stem Cells, Conditioned Medium, Metaplasia, Mitochondrial Transferمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.