Histological Study of The Effect of Chitosan Scaf-folds Loaded on The Different Concentrations of Hesperi-din Flavonoid in The Rat Model of Femoral Fracture
- سال انتشار: 1399
- محل انتشار: بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
- کد COI اختصاصی: RROYAN21_029
- زبان مقاله: انگلیسی
- تعداد مشاهده: 367
نویسندگان
Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
چکیده
Objective: Despite the success of bone graft in the healing of fracture, it has several limitations relating to donor-site mor-bidity, infection, bleeding, nerve damage and chronic pain. For that, the alternative biomaterials as scaffolds should be made with major influences on cell adhesion, proliferation and dif-ferentiation. Hesperidin, citrus flavonoid, inhibits bone loss by decreasing osteoclast formation and function. In this research, chitosan scaffolds with different concentrations of Hesperidin in rat femur defect repair were investigated.Materials and Methods: Thirty adult male Wistar rats weigh-ing ۲۰۰-۲۵۰ g were used and after creating fracture line on the right femur, they were divided randomly into six groups: con-trol (femoral fracture model), ۵ groups treated with chitosan scaffold with different concentrations of Hesperidin (۰, ۰.۰۱, ۰.۱, ۱ and ۱۰%). The chitosan scaffolds were prepared from solutions with chitosan (at concentration of ۱۲۰۰mg/۶۰cc ace-tic acid۱%) and evaluated wettability, porosity, degradation and compatibility by MTT, SEM and FTIR. After ۴ month, animals were sacrificed and the lesiond femurs extracted and stained with Hematoxylin and eosin (HandE) and Masson's trichrome (MT).Results: The results of MTT showed that the scaffold has no toxic effects on stromal cells. SEM and FTIR results showed the improvement of cell-scaffold interactions. Histological and CT-Scan studies showed that the defect in treated groups (۱ and ۱۰%) was fully replaced by new bone and connective tis-sue compared to control and Hesperidin (۰,۰.۰۱, ۰.۱%) treated groups. A stable collagenous matrix suitable for the osteoblastic proliferation and differentiation was formed in Hesperidin ۱۰% treated group compared to control.Conclusion: It seems that the chitosan scaffold loaded with ۱ and ۱۰% of Hesperidin has a potential role in the healing pro-cess bone lesions and it can be suitable as a therapeutic strategy in tissue engineering.کلیدواژه ها
Tissue Engineering, Scaffold, Chitosan, Hesperidin, Fractureمقالات مرتبط جدید
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