Study of Gene Expression Profile in Non-Invasive and Invasive Retinoblastoma
- سال انتشار: 1399
- محل انتشار: بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
- کد COI اختصاصی: RROYAN21_026
- زبان مقاله: انگلیسی
- تعداد مشاهده: 326
نویسندگان
Department of Brain and Cognitive Sciences, Cell Science Re-search Center, Royan Institute for Stem Cell Biology and Technol-ogy, ACECR, Tehran, Iran
Department of Brain and Cognitive Sciences, Cell Science Re-search Center, Royan Institute for Stem Cell Biology and Technol-ogy, ACECR, Tehran, Iran
Department of Ophthalmology, Ophthalmic Research Center,Torfe Hospital, Shahid Beheshti University of Medical Sciences,Tehran, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology andTechnology, ACECR, Tehran, Iran
Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Brain and Cognitive Sciences, Cell Science Re-search Center, Royan Institute for Stem Cell Biology and Technol-ogy, ACECR, Tehran, Iran
چکیده
Objective: Retinoblastoma (RB) is a malignant intraocular cancer of childhood with around ۹,۰۰۰ new cases a year. There are two kinds of RB: non-invasive and invasive, generally the later goes to morbidity and mortality due to secondary tumors. Family history in RB۱ gene mutation and paternal age play a significant role in RB invasion. RB late diagnosis cause to more enucleation in developing countries. The discovery of bi-omarkers and important signaling pathways for discrimination between invasive and non-invasive phases is necessary. In this study, by using the bioinformatics approach, we investigated the gene expression and microRNA profiling to find the dif-ferentiating biomarkers concerning non-invasive and invasive phases.Materials and Methods: We have specified the differentially expressed genes (DEGs) extracted from microarray librar-ies of non-invasive and invasive RB (GSE۹۷۵۰۸). Also, two miRNAs datasets in serum (GSE۴۱۳۲۱) and tissue (GSE۷۰۷۲) from GEO database and in-silico analysis were performed us-ing enrichment databases such as EnrichR, KEGG, Panther, TargetScan and STRING on signaling pathway, gene ontology, and protein and miRNAs networks.Results: Our results show that, ۱۸۴ genes were significantly downregulated and ۱۲۷ genes upregulated in invasive group compared to non-invasive. NTNG۱ and COL۴A۱ were selected as upregulated genes due to their presence in extracellular ma-trix and GUCA۱C and ARR۳ as downregulated genes. Among the expression profile of miRNAs in serum and tissues, miR-۲۰a and -۲۰۴, show the more accurate evaluations because they both are involved in MAPK, VEGF, TNF, Ras signaling and Cell adhesion molecules Which are associated with invasion retinoblastoma Conclusion: Using comprehensive bioinformatics analyzes, we could identify some genes and miRNAs as biomarkers in invasive and non-invasive retinoblastoma. Selected miRNAs by this study can be considered as biomarkers for improving the early detection of retinoblastoma.کلیدواژه ها
Retinoblastoma, Non-Invasive, Invasive, Biomarker, miRNAsمقالات مرتبط جدید
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