Simultaneous Treatment of Photobiomodulation and A Hybrid Graft of Seeded Human Demineralized Bone Ma-trix with Adipose-Derived Stem Cells Improve Osteogenesis in An Experimental Model of Critical-Sized Osteoporotic Defect

Objective: This research aimed to test the impact of simultane-ous treatment of Photobiomodulation (PBM) and hybrid BTE scaffold on accelerating the healing rate of a critical-size femo-ral defect (CSFD) in an osteoporotic rat model.Materials and Methods: The rats with CSFD were arbitrary separated into ۶ groups: control, Scaffold (S, DBM), S + PBM, S + alendronate (ALN), S + ASCs, and S + PBM + ASCs. Each group was assessed by Cone Beam Computed Tomography (CBCT) and histological examinations.Results: At the ۴th week, the largest volume of new bone formed in the S + PBM and S + PBM + ASC groups. S + PBM treatment relative to S and S + ALN treatments remarkably re-duced the defect area. S + PBM + ASCs treatment compared to the S and S + ALN treatments significantly decreased defect area. At the ۸th week, the defect area in the S + PBM group was substantially smaller than S, S + ALN and S + ASCs groups. Histological observations showed more new bone formation in the treated defects of S + PBM + ASCs and S + PBM groups. Conclusion: We conclude that the simultaneous treatment of osteoporotic rats with PBM plus DBM with or without seeded ASCs enhances bone healing.

کلیدواژه ها:

Photobiomodulation ، Demineralized Bone Matrix ، Human Adipose-Derived Stem Cells