Characterizations of Molecularly Distinct Subpopu-lations of Evs: In Vitro and In Vivo Studies
- سال انتشار: 1399
- محل انتشار: بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
- کد COI اختصاصی: RROYAN21_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 155
نویسندگان
Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology,ACECR, Tehran, Iran
چکیده
Recently, the possibility of replacing cells or liposomes with the naturally secreted micro-and nano scale extracellular vesi-cles (EVs) has sparked a heated debate. With its capacity to be used as therapeutics agent, cell conditioned medium derived EVs (CM-EVs) play an explosive role in the realm of regenera-tive medicine or pharmaceutical science. Increasing and com-pelling evidence additionally render EVs potentially promising options to be used as “commercial off-the-shelf product”. Ac-cepting the heterogeneity in EV subpopulations isolated by dif-ferent methods, in this report, following the isolation of EVs by different methods (ultracentrifuge, sucrose gradient, and ۲۰,۰۰۰ g centrifugation only), we confirmed that all of them met MISEV۲۰۱۸ criteria (size, morphology, and presence of ۳ positive markers and absence of ۱ negative marker). Further assessments has been done to find out the purity (based on albu-min contaminant), vesicular membrane perfectness (phosphati-dylserine exposure), as well as their functions in in-vitro tests (cellular uptake and proliferation), ex vivo test of angiogenesis and in vivo administration to monkey model of diabetes. Large scale proteome analysis of EVs sub-populations has also pro-vided us with some important clues to choose between tests and functions. In conclusion, we demonstrate that molecularly dis-tinct EV subpopulations can be evaluated by quantifiable meth-ods. Further assessments to correlate some functions to each subpopulation prior to designing clinical trials can accelerate the development of EV-based therapeutics.کلیدواژه ها
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