Aioub Sofizadeh - Center for Infectious Diseases Research, Golestan University of Medical Sciences, Gorgan, Iran.
Berdi Eftekhari - BSc in Public Health, Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Ali Reza Pesaraklo - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Ali Mohammadnia - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Farhad Ajam - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Morteza Farrokhi Balajadeh - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Mahmoud Yapang Gharavi - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.
Nader Sanad Gol - Health Centers in Golestan Counties, Golestan Province, Golestan University of Medical Sciences, Gorgan, Iran.

Background & Objectives: Mosquitoes (Diptera: Culicidae) are medically considered as one of the most important species of insects in terms of disease transmission to human beings; hence, they can be vectors of remarkable diseases such as malaria, West Nile, dengue fever, yellow fever, and filariasis. This study aimed to determine the species diversity and larval habitat characteristics of mosquitoes (Diptera: Culicidae) in Golestan Province in ۲۰۱۶. Materials & Methods: In this descriptive cross-sectional study, larval habitats of mosquitoes were investigated in ۱۴ counties of Golestan Province. Samples were collected by a standard ladle used for entomology. The collected larvae in lactophenol solution were transferred to a medical entomology laboratory in Gonbad-e Qabus County, and then microscopic slides were prepared. Mosquitoes species were identified based on their morphologic attributes and authentic identification keys. Larval habitat characteristics were studied in terms of the habitat type (permanent or temporary), water conditions (clear or turbid, stagnant or running), vegetation (with or without vegetation), sunlight exposure (full or partial exposure), and others. Data was analyzed using IBM SPSS software version ۱۸. Results: On the basis of analysis, ۲۸۹۱ third and fourth instars larvae, ۴ genera (Anopheles, Culex, Culiseta, and Ochlerotatus), and ۹ species (Anopheles superpictus, Culex mimiticus, Culex perexiguus, Culex pipiens, Culex pusillus, Culex theileri, Culex tritaeniohynchus, Culiseta longiareolata, Ochlerotatus caspius) of mosquitoes were detected, with the Culex pipiens being recognized as the dominant species of this family in Golestan Province. Conclusion: Due to the high species diversity of Culicidae in Golestan Province, further studies are of essence to investigate the ecology of medically important species such as Culex pipiens, Culex tritaeniorhynchus, and Anopheles superpicts, which are the vectors of many diseases in Golestan province.

Culicidae, Larval habitat, Species diversity, Ecology, Golestan