Crocin ameliorates MicroRNAs-associated ER stress in type ۲ diabetes induced by methylglyoxal
- سال انتشار: 1401
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 2
- کد COI اختصاصی: JR_IJBMS-25-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 160
نویسندگان
Student Research Committee, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Anatomical Sciences, School of Medicine, Medical Basic Sciences Research Institute, Cellular and molecular research center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Objective(s): Methylglyoxal (MG) provokes endoplasmic reticulum (ER) stress in β-cells and triggers pancreatic β-cell dysfunction. Crocin has anti-diabetic properties. The present study investigated whether crocin prevented pancreas damages induced by MG.Materials and Methods: Diabetes was induced by MG administration (۶۰۰ mg/kg/day, PO). On the fourteenth day, after proving hyperglycemia, crocin (۱۵, ۳۰, and ۶۰ mg/kg) and metformin (MT) (۱۵۰ mg/kg) were used for detoxification of MG until the end of the experiment. The animals were divided into ۶ groups: ۱) control, ۲) diabetic by MG, ۳) MG + crocin ۱۵ mg/kg, ۴) MG + crocin ۳۰ mg/kg, ۵) MG + crocin ۶۰ mg/kg, and ۶) MG + MT. The data were analyzed by one-way analysis of variance and significant differences were compared by Tukey and Bonferroni tests (P< ۰.۰۵). Biochemical assays, antioxidant evaluation, and microRNAs expression associated with ER stress were assessed.Results: MG induced hyperglycemia, insulin resistance, and dyslipidemia (P< ۰.۰۰۱). Crocin and MT significantly ameliorated β-cell function through reduction of fasting blood glucose, malondialdehyde levels (P< ۰.۰۰۱), and significant elevation of anti-oxidant enzyme activity accompanied by regulation of glutathione and glyoxalase۱-Nrf۲ in MG induced diabetic mice. Crocin and MT significantly down-regulated microRNAs ۲۰۴, ۲۱۶b, ۱۹۲, and ۲۹a expression (P< ۰.۰۰۱). Crocin (۶۰ mg/kg) (P< ۰.۰۱) and MT (P< ۰.۰۰۱) could improve diameter of pancreatic islets in MG treated mice. Conclusion: Crocin prevents the progression of diabetes through modulating ER stress-associated microRNAs and GLO۱ activity with the helpful effects of glutathione and Nrf۲.کلیدواژه ها
Crocin, Diabetes, ER stress, Glyoxalase ۱, Methylglyoxal, miR-۲۰۴اطلاعات بیشتر در مورد COI
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