Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model

  • سال انتشار: 1401
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 2
  • کد COI اختصاصی: JR_IJBMS-25-2_003
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 235
دانلود فایل این مقاله

نویسندگان

Luis Miguel Paco Meza

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

MDolores Carmona

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

Sagrario Canadillas

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

Ana Lopez-Diaz

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

Francisco Munoz-Lopez

Bio-Knowledge Lab, Glorieta de los Países Bálticos, s/n. Edificio Baobab ۱, Oficina ۱۵, Polígono Tecnocórdoba, ۱۴۰۱۴ Córdoba, Spain

Alvaro Jimenez-Arranz

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

Ipek Guler

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

Concha Herrera

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP ۱۴۰۰۴ Córdoba, Spain

چکیده

Objective(s): Adipose tissue-derived mesenchymal stromal cells (ASCs) are useful in cell-based therapy.  However, it is well known that diabetes mellitus (DM) alters ASCs’ functionality. The majority of in vitro studies related to ASCs are developed under non-physiological oxygen conditions. Therefore, they may not reflect the full effects of DM on ASCs, in vivo. The main aim of the current study is to identify molecular pathways and underlying biological mechanisms affected by diabetes on ASCs in physiological oxygen conditions.Materials and Methods: ASCs derived from healthy (ASCs-C) and diabetic (ASCs-D) rats were expanded under standard culture conditions (۲۱% O۲) or cultured in physiological oxygen conditions (۳% O۲) and characterized. Differential gene expressions (DEGs) of ASCs-D with respect to ASCs-C were identified and analyzed with bioinformatic tools. Protein-protein interaction (PPI) networks, from up- and down-regulated DEGs, were also constructed.Results: The bioinformatic analysis revealed ۱۳۵۴ up-regulated and ۸۵۹ down-regulated DEGs in ASCs-D, with ۲۱ and ۷۸ terms over and under-represented, respectively. Terms linked with glycosylation and ribosomes were over-represented and terms related to the activity of RNA-polymerase II and transcription regulation were under-represented. PPI network disclosed RPL۱۱-RPS۵ and KDR-VEGFA as the main interactions from up- and down-regulated DEGs, respectively.Conclusion: These results provide valuable information about gene pathways and underlying molecular mechanisms by which diabetes disturbs ASCs biology in physiological oxygen conditions. Furthermore, they reveal, molecular targets to improve the use of ASCs in autologous transplantation.

کلیدواژه ها

Cell-based therapy, Diabetes, Enrichment analysis, Microarray, Physioxia

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.