Whole-Genome Sequencing of a Clinically Isolated Antibiotic-Resistant Enterococcus faecium EntfacYE
- سال انتشار: 1400
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 15، شماره: 6
- کد COI اختصاصی: JR_IJMM-15-6_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 230
نویسندگان
Department of Genetics, Faculty of Life Sciences, Islamic Azad University Tehran North Branch, Tehran, Iran
Pasteur Institute, Tehran, Iran
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background and Objective: Enterococcal infections are considered the most common nosocomial infections. Nowadays, enterococci show high resistance to common antibiotics, especially vancomycin. Vancomycin-resistant Enterococcus faecium is one of the most common nosocomial infections, which is included in the World Health Organization priority pathogens list for research and development of new antibiotics. In this case, we focused on the E. faecium EntfacYE genome and its antibiotic-resistant genes to understand the reasons that caused this bacteria to be resistant to antibiotics. Materials and Methods: In total, ۲۵ enterococcal samples were isolated from patients' blood. Bacteriophages were isolated on a multidrug-resistant Enterococcus faecium EntfacYE in our previous study. In this study, the isolated E. faecium EntfacYE strain was verified using Sanger partial sequencing of the bacterial elongation factor Tu. EntfacYE strain was assessed for antibiotic resistance, and the bacterial genome was extracted and completely sequenced. The sequenced genome was analyzed, and the genes were annotated in the DNA Data Bank of Japan. Results: Totally, EntfacYE genome subsystems included ۲۳ various categories with ۵۹ genes belonging to antimicrobial resistance genes, such a way that ۴۹ antibiotic resistance genes were included in specific subsystems, while ten genes lacked specific subsystems. Moreover, cadmium, cobalt, copper, zinc, and mercury resistance genes were identified in the EntfacYE genome. Conclusion: In conclusion, studies on bacterial genomes help researchers to identify characteristics of common pathogens, including virulence and antibiotic-resistance genes, and hence better understand bacterial pathogenesis to provide novel solutions for the treatment of common infections.کلیدواژه ها
Antibiotic resistance, Clinical sample, Enterococcus faecium, Whole-genome Sequencing, توالی یابی کامل ژنوم, انتروکوکوس فاسیوم, مقاومت آنتی بیوتیکی, نمونه بالینیاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.