Preparation and Evaluation of a Niosomal Drug Delivery System Containing Cefazolin and Study of Its Antibacterial Activity

Background and Objective: Infectious diseases are one of the leading causes of death in the world. The use of antibiotics, in addition to limitations and side effects, causes the resistance of microorganisms. The use of drug delivery systems is an effective way to increase drug stability and reduce antibiotic use. This study aimed to load cefazolin into a niosomal drug delivery system. Materials and Methods: This study was performed in ۲۰۱۸ to investigate the effect of span ۶۰, tween ۶۰, and cholesterol on the synthesis of niosome nanoparticles loaded with cefazolin by the thin layer hydration method. Then the characteristics of synthetic niosome nanoparticles and their antibacterial activity were investigated. Results: SEM images showed that all nanoparticles are spherical. The encapsulation efficiencies for the first, second, and third formulations were ۳۳%, ۱۹.۷%, and ۴۰.۷۶%, respectively. The release of cefazolin from the first, second, and third formulations during ۳۰ days was ۴۸%, ۸۱.۵%, and ۶۳%. The particle size and zeta potential of the third niosome formulation were estimated to be ۱۵۴ nm and -۲۴ mV. The MIC for Escherichia coli and Staphylococcus aureus were ۴ and ۱۵۰ μg/mL, respectively. The niosome nanoparticles prepared with the third formulation show an excellent antibacterial effect against Escherichia coli for six days, and the diameter of its growth inhibition zone remains almost constant. Conclusion: The optimal formulation of niosome nanoparticles included span ۶۰ (۰.۰۶۰), tween ۶۰ (۰.۰۹۰), and cholesterol (۰.۰۴۶). Continuous and controlled release of cefazolin from the niosome, along with increased drug penetration, reduces the growth of E. coli (ATCC ۹۶۳۷) and S. aureus (ATCC ۱۲۶۰۰).

کلیدواژه ها:

Antibiotic ، Cefazolin ، Controlled drug delivery ، Infectious diseases ، Niosome ، نیوزوم ، آنتی بیوتیک ، سفازولین ، بیماری های عفونی ، دارورسانی کنترل شده