Research Article: Molecular diagnosis of pathogenic Aeromonas hydrophila in Cyprinus carpio ulcers
- سال انتشار: 1399
- محل انتشار: مجله علوم شیلات ایران، دوره: 19، شماره: 6
- کد COI اختصاصی: JR_JIFRO-19-6_028
- زبان مقاله: انگلیسی
- تعداد مشاهده: 208
نویسندگان
Fish Recourses and Aquatic Animals Department, College of Agriculture, Salahaddin University-Erbil, Erbil, Iraq.
Fish Recourses and Aquatic Animals Department, College of Agriculture, Salahaddin University-Erbil, Erbil, Iraq.
Biology Department, College of Science, Salahaddin University-Erbil, Erbil, Iraq.
چکیده
A total of ۱۵۰ carp fish were collected from Grdarasha Farm (۸ kilometers from Erbil-Iraq) and examined for the presence of pathogenic Aeromonas hydrophila on their skin ulcers, this bacterium secretes a toxin protein called aerolysin which is a virulence factor and has a role in diarrhoeal diseases, deep wound infections and hemorrhagic septicemia. The present study aimed to determine the presence of A. hydrophila in common carp (Cyprinus carpio). For this purpose samples were collected from fish ulcer by swap and incubated at ۳۷°C in blood agar to determine the shape and morphology of colonies, and then the colonies were examined by gram stain test for microscopic examination, finally molecular diagnosis was conducted for aerolysin gene with primer design and PCR. According to the diagnosis methods results were different; PCR diagnosis confirmed that only ۶۸ skin lesions were caused by A. hydrophila, no visceral lesion was caused by A. hydrophila and this bacterium hardly ever seen in the skin surrounding mouth as only ۲ lesions surrounding mouth had A. hydrophila in PCR diagnosis.کلیدواژه ها
Aerolysin, Aeromonas hydrophila, Cyprinus carpio, Polymerase chain reactionاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.