Research Article: Molecular phylogeny of bivalve families (Arcidae, Chamidae, Margaritidae, Ostreidae, Veneridae) in the Persian Gulf

  • سال انتشار: 1399
  • محل انتشار: مجله علوم شیلات ایران، دوره: 20، شماره: 1
  • کد COI اختصاصی: JR_JIFRO-20-1_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 311
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نویسندگان

Sh. Masaeli

Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran/Iran

P. Ghavam Mostafavi

Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran/Iran

H. Hosseinzadeh Sahafi

Iranian Fisheries Science Research Institute Agriculture Research , Education and Extension Organization. Tehran, Iran

S. Tamadoni Jahromi

Persian Gulf and Oman Sea Ecology Research center , Iranian Fisheries Science Research Institute , Agricultural Research Education and Extention Organization (AREEO) , Bandar Abass.Iran

A. Nabinejad

Veterinary Department , Isfahan Research and education center of agriculture , Amir hamzeh city , Isfahan , Iran

V. Noaman

Department of Animal Parasitic Disease, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), karaj, Iran

چکیده

Bivalvia is one of the least studied classes of the Persian Gulf. A survey and molecular analysis was conducted to determine bivalve species diversity in the Persian Gulf. To the best of our knowledge, this is the first report of barcoding data on bivalves of the Persian Gulf. We examined ۴۰ individuals representing ۸ species, ۶ genera and ۵ families. We collected samples from Hengam, Larak and Qeshm Islands and Bandar Lengeh in Persian Gulf, Iran. After DNA extraction, mitochondrial ۱۶S ribosomal DNA (۱۶SrDNA) and Cytochrome Oxidase subunit I were amplified by polymerase chain reaction (PCR). Based on obtained ۱۶SrDNA and COI gene sequences and maximum parsimony, neighbor joining and maximum likelihood trees of these genes there was no overlap between maximum Kimura ۲- parameter distance among conspecifics. Most species formed agglutinate sequence units with a small amount of changes. Eventually, comparison of the ۸ selected studied species with metadata from India, Brazil, Japan, China, and America exposed that these species in Persian Gulf are classified in sister clades with high bootstraps except Pinctada. Since there is not much work on bivalves identification in the Persian Gulf, larger sampling and more research is needed to investigate mollusc diversity in this area.

کلیدواژه ها

Bivalvia, Persian Gulf, ۱۶SrDNA, DNA sequencing, COI

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