Evaluation of the Diagnostic Value of Calretinin Immunohistochemistry Assay in the Superficial Rectal Biopsy of Children Suspected of Hirschsprung's Disease

  • سال انتشار: 1401
  • محل انتشار: مجله علمی ناباروری ایران، دوره: 13، شماره: 1
  • کد COI اختصاصی: JR_IRJN-13-1_005
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 138
دانلود فایل این مقاله

نویسندگان

Azar Naimi

Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran

Elahe Shegeft

Isfahan University of Medical Sciences, Isfahan, Iran

چکیده

Background: Hirschsprung's disease (HD) is the most common cause of colon obstruction in neonates. Lack of calretinin immunohistochemistry (IHC) in colon lamina propria could be associated with aganglionosis. Methods: This cross-sectional retrospective study examined ۶۴ patients suspicious of HD and conducted on collected-demographic data and Hematoxylin/Eosin (H & E) results of the surgical rectal biopsy from the archive of Imam Hossein Hospital, Isfahan, Iran. Calretinin IHC was achieved for all the specimens, and the results of the two methods were compared. The receiver operating characteristic (ROC) chart shows sensitivity changes according to (۱-specificity). Moreover, sensitivity, specificity, as well as the positive and negative predictive values (PPV and NPV) of calretinin staining in lamina propria and muscularis mucosa, compared to H & E staining, was calculated in this study. Results: The number of males and females was the same in this study (n=۳۲; ۵۰%). The age of the patients ranged from ۱ month to ۱۵۶ months (۱۳ years). Out of ۶۴ patients, ۸ cases were diagnosed with HD, and calretinin staining was negative in ۷ patients; moreover, only one case showed weak positive staining. In ۵۶ patients, who were not HD, two patients with negative calretinin staining had a positive H&E staining report. The current study aimed to check the diagnostic value of calretinin IHC in lamina propria of patients suspicious of HD. Compared to H&E staining, which is the routine method in thick wall biopsies, the statistics show that calretinin IHC has a high diagnostic value (P< ۰.۰۰۱), sensitivity of ۸۷%, specificity of ۹۶%, PPV of ۷۷%, and NPV of ۹۸%. Conclusion: In conclusion, calretinin IHC utilized in specimens seems superficial and inadequate; moreover, the association with the high specificity percentage (۹۶%) could help physicians with less invasive biopsy methods, such as endoscopic rectal biopsy.    

کلیدواژه ها

Calretinine, immunohistochemistry, Hirschsprung, rectal biopsy

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.