Natural killer cell derived Extracellular Vesicles: promising candidate in cancer therapy
- سال انتشار: 1400
- محل انتشار: پنجمین کنگره بین المللی سرطان
- کد COI اختصاصی: CANCERMED05_135
- زبان مقاله: انگلیسی
- تعداد مشاهده: 267
نویسندگان
Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan Unive
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
چکیده
Introduction: Ovarian cancer is the fifth most lethal cancer in women. According to the low efficiency of common therapies and also high rate of cancer recurrence, developing a new effective therapy is greatly in demand. Natural Killer Cells and Natural Killer Cell derived Extracellular Vesicles (NK-EVs) have therapeutic potential in cancer therapy. Therefore, in this study we have evaluated cytotoxic effect of natural killer cell derived extracellular vesicles on SKOV۳ cells in ovarian cancer. Methods: First, NK cells were activated by adding IL۱۵ (۱۰ ng/ml). Then, culture supernatant was harvested after ۲۴ h by centrifugation at ۲۰۰۰ rpm for ۵ min to remove cells. Different fractions of NK-EVs were isolated by differential centrifugation at ۲۰۰۰g, ۱۰۰۰۰g and ۲۰۰۰۰g respectively. SKOV۳ cells were treated with NK-EVs. Cytotoxicity of the NK-EV was evaluated using MTS assay. The study groups were ۲۰۰۰g NK-EV treatment group, ۱۰۰۰۰g NK-EV (large EV/lEV) treatment group, ۲۰۰۰۰g NK-EV (medium EV/mEV) treatment group, NK cell derived conditioned medium and control group. Results: According to MTS results, mEVs have the highest cytotoxic effect on SKOV۳ cells. Conclusion: NK-EVs have cytotoxic effect on SKOV۳ cells in vitro. NK-EV is considered as an off-the-shelf product and easy to use and store for a long time. Therefore, it could be a great cancer therapy strategy in the future.کلیدواژه ها
Ovarian cancer, SKOV۳ cell line, Natural Killer cell derived Extracellular Vesiclesمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.