Evaluate the effect of ATRA and hydroalcoholic extract of Dandelion alone and also their combined effect on the expression of genes that they have a critical role in proliferation and invasion of MCF-۷ breast cancer

Introduction: The present study sought to evaluate the effect of ATRA and hydroalcoholic extract of Dandelion alone, and combined, on the expression of p۵۳, Matrix Metalloproteinase -۲ (MMP-۲), and Matrix Metalloproteinase- ۹ (MMP-۹) genes and their role in proliferation and invasion of MCF-۷ breast cancer cells. Methods: MCF-۷ cells were cultured in a ۹۶-well plate with a density of ۵۰۰۰ cells per well. After ۲۴ hours, MCF-۷ cells were exposed to increasing concentrations of dandelion extract, ATRA, or the combination of dandelion extract and ATRA (in the same concentration ranges) to determine cell viability. Results: The extract of dandelion alone or in combination with ATRA induced cytotoxic effects in the concentration range below IC۵۰, a significant decrease expression gene of MMP-۹ as well as an increase expression gene of p۵۳ compared to the control group (p<۰.۰۰۱). MMP-۲ expression in ATRA, dandelion extract, or the combination of dandelion extract and ATRA-treated groups was significantly higher compared to the control group (p˂۰.۰۰۱). Conclusion: Dandelion or its combination with ATRA, through upregulation of p۵۳ expression and simultaneously downregulation of MMP-۹ expression in MCF-۷ cancer cells, can affect proliferation and metastasis processes in these malignant cell lines.

کلیدواژه ها:

All-trans retinoic acid (ATRA) ، breast cancer ، dandelion ، gene expression