The Synergistic Cytotoxic and Apoptotic Effect of Resveratrol and Naringenin on Y۷۹ Retinoblastoma Cell Line

Introduction: Resveratrol is a phenolic natural product which is found in red grapes also in Japanese knotweed root (Polygonum cuspidatum). Naringenin is one of the flavonoid compounds found in landing grape and other citrus fruits. Both agents exert antioxidant and anti-inflammatory properties. In this study, we investigated the effect of Resveratrol and Naringenin in an in vitro model of retinoblastoma of the eye. Method: XTT and trypan blue assays used to evaluate the anti-proliferative/cytotixic effect of resveratrol and naringenin in Y۷۹ cells. By the aid of AnnexinV/PI flow cytometry the kind of cell death investigated. To asses important gene expression level at mRNA level involve in apoptosis, Real-time PCR utilized. Results: Naringenin and resveratrol significantly decreased proliferation and stimulated cell death (mostly apoptosis) in Y۷۹ cells at ۵۰ and ۱۰۰ (μg/ml) after ۲۴ and ۴۸ hours. More cytotoxic effect observed after ۴۸ hours. Furthermore expression level of Bax and Bcl۲ mRNAs altered significantly in all samples treated with ۵۰ (μg/ml) of naringenin, resveratrol, or simultaneously with both. P۲۱ mRNAs expression altered in all mentioned samples except those treated with ۵۰ (μg/ml) of resveratrol. Conclusion: Based on the results, it can be concluded that resveratrol and naringenin can decrease cell viability in retinoblastoma cells in an in vitro dose/time-dependent manner. Albeit more studies needed to shed the light on the mechanism of action, our data reveals a potential synergistic cytotoxic effect of naringenin and resveratrol on Y۷۹ cells in ۴۸ hours.

کلیدواژه ها:

Resveratrol ، Naringenin ، Retinoblastoma ، Synergism ، Cytotoxic ، Y۷۹ cell line