Biosynthesis of Silver Nanoparticles Using Hydroethanolic Extract of Cucurbita pepo L. Fruit and Their Anti-proliferative and Apoptotic Activity Against Breast Cancer Cell Line (MCF-۷)

  • سال انتشار: 1400
  • محل انتشار: مجله تحقیقات سرطان، دوره: 5، شماره: 3
  • کد COI اختصاصی: JR_MCIJO-5-3_004
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 156
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نویسندگان

Leila Soltani

Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

Maryam Darbemamieh

Department of Plant Protection, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

چکیده

Introduction: Cancer is the second leading cause of death all over the world and breast cancer is the second common member of cancers worldwide. In this study, silver nanoparticles (Ag-NPs) were synthesized; using hydroethanolic extract of Cucurbita pepo L. fruit and evaluated for their antiproliferative and apoptotic activities against MCF-۷ cell line. Methods: Ag-NPs formation was characterized by ultraviolet-visible spectroscopy, energy dispersive spectroscopy (EDX), scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier transform infrared (FTIR) spectroscopy. Cytotoxicity and apoptosis responses were evaluated by ۳-[۴,۵-dimethylthiazole-۲-yl]-۲,۵- diphenyltetrazolium bromide (MTT) and dual acridine orange/ethidium bromide (AO/ EB) fluorescent staining, respectively. Results: Our results demonstrated the formation of Ag-NPs by Cucurbita pepo L. fruit extract discoloration to dark black. This transformation revealed their slightly aggregated shapes to quasi-spherical form with a mean diameter of ۱۰۴ nm. The zeta potential value was -۴۲.۳ mV for any Ag-NPs. These results indicated the successful formation of Ag- NPs for cellular uptake. MTT results showed that Ag-NPs significantly decreased the viability and induced MCF-۷ cells apoptosis in a dose-dependent manner; especially at concentrations of ۵۰ and ۲۵۰ μg/mL. Conclusions: In conclusion, according to the results of the present study, biologically synthesized Ag-NPs induce apoptotic and cytotoxic effects against breast cancer cell lines in a dose-dependent manner.

کلیدواژه ها

Cell Line, Breast Neoplasms, Nanoparticles, Green Chemistry Technology, Cucurbita

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